Mispa Lume

Mispa Lume

Real Time Isothermal LAMP Analyzer

Overview

Mispa Lume is a highly compact molecular diagnostics system to simultaneously perform 16 samples at faster TAT for real time detection of target genes using RT-LAMP reaction.

Mispa Lume – Top Ranked RT-LAMP Analyzer

Mispa Lume is a very small molecular diagnostics machine that can run 16 samples simultaneously at a quicker TAT for real-time target gene identification utilizing RT-LAMP reaction.

What is LAMP Technology:

LAMP is the short form for Loop-mediated isothermal amplification, a gene amplification assay for detecting the target gene. LAMP is faster than traditional PCR at analyzing genetic material. Reverse-transcription LAMP (RT-LAMP) amplifies RNA gene targets and has been used to find the COVID-19 virus. RT-LAMP with crude RNA samples can be used for quick testing at the point of care because it can use patient samples directly. It is crucial to select a diagnostic technique that is simpler and quicker than RT-PCR, depending on the circumstance. Both RT-LAMP and LAMP use four to 6 primers that recognize the target DNA. A strand-displacing DNA polymerase starts the process, and 2 of the primers form loop structures that make it easier for the next amplification round.

Comparison of RT-LAMP with other technologies for the Detection of SARS-CoV-2

RT-LAMP, as discussed, is a simple, sensitive, quick, and cost-effective method that can find pathogens early. The reagents storage is between 2-8*C, and better performance is reported from established lab validations. InstaLume and SARS-Cov-2 are suitable reagents for very less TAT, and you get the results in 35 minutes post-sampling.

Mispa Lume gives accurate results with 100% specificity, 99.3% accuracy, and 98.7% sensitivity for Covid-19 confirmatory test. LAMP machine is available with affordable investment in smaller labs. Any smaller labs with less turnover also can use this machine with limited test numbers at a time.

Features:

  • One tube & multiple target genes
  • Results within minutes after extraction 
  • Positive result can be detected from 10 minutes onwards
  • 6 primers per target for 100% specificity
  • Real-Time lamp assay with 98.7% sensitivity 
  • Automatic and faster ct analysis 
  • 2°C to 8°C storage/ transportation of kits
  • Performs 16 tests simultaneously

Technology:

RT-LAMP – Reverse Transcriptase Loop Mediated Isothermal Amplication

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